Rapid and simple liquid chromatography tandem mass spectrometry method for the quantification of zidovudine in rat plasma.

A high-performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the quantification of zidovudine in rat plasma. Following solid-phase extraction, the analytes were separated using an isocratic mobile phase on a reverse phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M+H]+ ions, m/z 268/127 for zidovudine and m/z 230/112 for the internal standard. The method exhibited a linear dynamic range of 5-500 ng/mL for zidovudine in rat plasma. The lower limit of quantification was 5 ng/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 1.5 min for each sample made it possible to analyze more than 400 plasma samples per day. The validated method was applied for pharmacokinetic studies of the novel drug delivery systems of zidovudine in rats.

Mudigonda K ，Jukanti R ，Apte SS ，Kishan V ，Maurya S ，Kandikere V ，Nirogi R ... -  《BIOMEDICAL CHROMATOGRAPHY》

Quantification of pseudoephedrine in human plasma by LC-MS/MS using mosapride as internal standard.

Nirogi RV ，Kandikere VN ，Shukla M ，Mudigonda K ，Maurya S ，Komarneni P ... -  《BIOMEDICAL CHROMATOGRAPHY》

Quantification of fexofenadine in human plasma by liquid chromatography coupled to electrospray tandem mass spectrometry using mosapride as internal standard.

A rapid high-performance liquid chromatography/positive ion electrospray tandem mass spectrometry method was developed and validated for the quantification of fexofenadine in human plasma using mosapride as internal standard. Following solid-phase extraction, the analytes were separated using an isocratic mobile phase on a reverse-phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M+H]+ ions, m/z 502/466 for fexofenadine and m/z 422/198 for the IS. The method exhibited a linear dynamic range of 1-500 ng/mL for fexofenadine in human plasma. The lower limit of quantification was 1 ng/mL with a relative standard deviation of less than 5% for fexofenadine. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The total chromatographic run time of 2 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies.

Nirogi RV ，Kandikere VN ，Shukla M ，Mudigonda K ，Maurya S ，Komarneni P ... -  《BIOMEDICAL CHROMATOGRAPHY》

Sensitive liquid chromatography tandem mass spectrometry method for the quantification of Quetiapine in plasma.

Nirogi R ，Bhyrapuneni G ，Kandikere V ，Mudigonda K ，Ajjala D ，Mukkanti K ... -  《BIOMEDICAL CHROMATOGRAPHY》

Quantification of pramipexole in human plasma by liquid chromatography tandem mass spectrometry using tamsulosin as internal standard.

A high-performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the quantification of pramipexole in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse-phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M + H](+) ions, m/z 212/152 for pramipexole and m/z 409/228 for the IS. The method exhibited a linear dynamic range of 200-8000 pg/mL for pramipexole in human plasma. The lower limit of quantification was 200 pg/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 3.5 min for each sample made it possible to analyze more than 200 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies.

Nirogi RV ，Kandikere V ，Shrivastava W ，Mudigonda K ，Maurya S ，Ajjala D ... -  《BIOMEDICAL CHROMATOGRAPHY》