Enhanced accumulation of phosphorylated alpha-synuclein in double transgenic mice expressing mutant beta-amyloid precursor protein and presenilin-1.

A recent report showed that the accumulation of alpha-synuclein (alpha-syn) was detected in the brains of one-third of Alzheimer's disease and Down syndrome patients. However, the relationship between amyloid-beta protein (Abeta) and alpha-syn remains unclear. We analyzed the relation between the mutation of presenilin-1 (PS-1) and the pathological features of beta-amyloidosis and alpha-synucleinopathy. We generated doubly transgenic mice overexpressing mutant beta-amyloid precursor protein (betaAPP; Tg2576) and mutant PS-1 (PS1L286Vtg; line 198) and analyzed 19 double Tg betaAPP(+)/PS(+) mice at 5-23 months (young to old), 23 age-matched single Tg betaAPP(+)/PS(-) mice, and 11 non-Tg littermates. Immunohistochemical comparison was performed in these three groups by counting the area and the number of alpha-syn- or phosphorylated alpha-syn (palpha-syn)-positive dystrophic neurites per plaque (ASPDN, pASPDN). The acceleration of Abeta pathology was found with earlier onset and exaggerated numbers in double Tg betaAPP(+)/PS(+) compared with single Tg betaAPP(+)/PS(-) mouse brains. The accumulation of ASPDN and pASPDN was also accelerated in double Tg betaAPP(+)/PS(+) compared with single Tg betaAPP(+)/PS(-) mouse brains, especially in pASPDN. The number and area of alpha-syn and palpha-syn, and the ratio of palpha-syn positive neurites were significantly higher in double Tg betaAPP(+)/PS(+) than in single Tg betaAPP(+)/PS(-) mouse brains in middle-aged and old groups. Additional overexpression of mutant PS-1 accelerated Abeta-induced alpha-synucleinopathy and further facilitated the phosphorylation of alpha-syn, suggesting a direct association between mutant PS-1 and phosphorylation of alpha-syn.

Kurata T ，Kawarabayashi T ，Murakami T ，Miyazaki K ，Morimoto N ，Ohta Y ，Takehisa Y ，Nagai M ，Ikeda M ，Matsubara E ，Westaway D ，Hyslop PS ，Harigaya Y ，Kamiya T ，Shoji M ，Abe K ... -  《JOURNAL OF NEUROSCIENCE RESEARCH》

Enhanced accumulation of tau in doubly transgenic mice expressing mutant betaAPP and presenilin-1.

Samura E ，Shoji M ，Kawarabayashi T ，Sasaki A ，Matsubara E ，Murakami T ，Wuhua X ，Tamura S ，Ikeda M ，Ishiguro K ，Saido TC ，Westaway D ，St George Hyslop P ，Harigaya Y ，Abe K ... -  《BRAIN RESEARCH》

Reduced retinal function in amyloid precursor protein-over-expressing transgenic mice via attenuating glutamate-N-methyl-d-aspartate receptor signaling.

Shimazawa M ，Inokuchi Y ，Okuno T ，Nakajima Y ，Sakaguchi G ，Kato A ，Oku H ，Sugiyama T ，Kudo T ，Ikeda T ，Takeda M ，Hara H ... -  《-》

Transgenic mice overexpressing human acetylcholinesterase and the Swedish amyloid precursor protein mutation: effect of nicotine treatment.

Hedberg MM ，Svedberg MM ，Mustafiz T ，Yu WF ，Mousavi M ，Guan ZZ ，Unger C ，Nordberg A ... -  《NEUROSCIENCE》

Exclusive association and simultaneous appearance of congophilic plaques and AT8-positive dystrophic neurites in Tg2576 mice suggest a mechanism of senile plaque formation and progression of neuritic dystrophy in Alzheimer's disease.

Noda-Saita K ，Terai K ，Iwai A ，Tsukamoto M ，Shitaka Y ，Kawabata S ，Okada M ，Yamaguchi T ... -  《ACTA NEUROPATHOLOGICA》