Chondrogenic differentiation of human mesenchymal stem cells in collagen type I hydrogels.

The chondrogenic differentiation of bone marrow-derived human mesenchymal stem cells (MSCs) in a collagen type I hydrogel, which is in clinical use for matrix-based autologous chondrocyte transplantation (ACT), was investigated. Collagen hydrogels with 2.5 x 10(5) MSCs/mL were fabricated and cultured for 3 weeks in a serum-free, defined, chondrogenic differentiation medium containing 10 ng/mL TGF-beta1 or 100 ng/mL BMP-2. Histochemistry revealed morphologically distinct, chondrocyte-like cells, surrounded by a sulfated proteoglycan-rich extracellular matrix in the TGF-beta1 and BMP-2 treated group, with more elongated cells seen in the BMP-2 treated group. Immunohistochemistry detected collagen type II (Col II) in the TGF-beta1 and BMP-2 treated group. Collagen type X (Col X) staining was positive in the TGF-beta1 but only very weak in the BMP-2 treated group. RT-PCR analyses revealed a specific chondrogenic differentiation with the expression of the cartilage specific marker genes Col II, Col X, and aggrecan (AGN) in the TGF-beta1 and the BMP-2 treated group, with earlier expression of these marker genes in the TGF-beta1 treated group. Interestingly, MSC-gels cultured in DMEM with 10% FBS (control) indicated few isolated chondrocyte-like cells but no expression of Col II or Col X could be detected. The results show, that MSCs cultured in a collagen type I hydrogel are able to undergo a distinct chondrogenic differentiation pathway, similar to that described for MSCs cultured in high-density pellet cultures. These findings are valuable in terms of ex vivo predifferentiation or in situ differentiation of MSCs in collagen hydrogels for articular cartilage repair.

Nöth U ，Rackwitz L ，Heymer A ，Weber M ，Baumann B ，Steinert A ，Schütze N ，Jakob F ，Eulert J ... -  《JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A》

Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: influence of collagen type II extracellular matrix on MSC chondrogenesis.

Bosnakovski D ，Mizuno M ，Kim G ，Takagi S ，Okumura M ，Fujinaga T ... -  《BIOTECHNOLOGY AND BIOENGINEERING》

Can TGF-beta1 and rhBMP-2 act in synergy to transform bone marrow stem cells to discogenic-type cells?

Kuh SU ，Zhu Y ，Li J ，Tsai KJ ，Fei Q ，Hutton WC ，Yoon ST ... -  《-》

Regulation of mesenchymal stem cell and chondrocyte differentiation by MIA.

Tscheudschilsuren G ，Bosserhoff AK ，Schlegel J ，Vollmer D ，Anton A ，Alt V ，Schnettler R ，Brandt J ，Proetzel G ... -  《EXPERIMENTAL CELL RESEARCH》

Adenovirus mediated BMP-13 gene transfer induces chondrogenic differentiation of murine mesenchymal progenitor cells.

Nochi H ，Sung JH ，Lou J ，Adkisson HD ，Maloney WJ ，Hruska KA ... -  《JOURNAL OF BONE AND MINERAL RESEARCH》