Prevalence of the most common high-risk HPV genotypes among women in three new independent states of the former Soviet Union.

Type distribution of HPV has been studied in different geographic regions, but the data are scanty from the new independent states of the former Soviet Union. Here the HPV prevalence and distribution of the most frequent high-risk HPV types among 3,187 women at different risk for HPV and cervical intraepithelial neoplasia in Russia, Belarus, and Latvia is reported. HPV detection, type distribution and viral load analysis in DNA samples from cervical scrapes were done with real-time PCR-based assay detecting HPV types 16, 18, 31, 33, 35, 39, 45, 52, and 58. The overall HPV prevalence was 31.2%, HPV16 was the most prevalent type followed by HPV31 and HPV33 group. The overall HPV prevalences in Russia, Belarus and Latvia were 33.4%, 27.5%, and 26.2%. The type distributions were similar in these countries, except for Latvia where HPV39 was the third prevalent genotype. HPV prevalence was highest (40.8%) among women from sexually transmitted disease clinic, followed by 30.9% among gynecological outpatients and 27.2% in screening patients. HPV detection increased with cytological abnormality (P = 0.0001) and lesion grade in the biopsy (P = 0.0001), from 27% to 72% in normal samples to cancer, and from 64% to 77% in cervical intraepithelial neoplasia 1 to cancer. The normalized viral loads varied greatly between and among different HPV-types. The mean log HPV33 group copies/cell increased from negative for intraepithelial lesions to cancer (P = 0.049). Distribution of the most common high-risk HPV-types seems to be similar in these countries as reported in other major geographical regions.

Kulmala SM ，Shabalova IP ，Petrovitchev N ，Syrjänen KJ ，Gyllensten UB ，Syrjänen SM ，NIS Study Group ... -  《JOURNAL OF MEDICAL VIROLOGY》

Prevalence and type distribution of high-risk human papillomavirus infection in women undergoing voluntary cervical cancer screening in Italy.

The aim of this survey was to assess the prevalence and distribution of oncogenic human papillomavirus (HPV) genotypes in women who underwent screening for cervical cancer in Italy. The correlation of genotypes with the cytological results was also evaluated. Cervical samples were collected from 9,947 self-referring women for cervical cancer screening. Participants were screened by liquid-based cytology and high-risk HPV testing using the Hybrid Capture 2 test. Positive samples were genotyped by PCR. Samples (1,474; 14.8%) were positive for high-risk HPV. The prevalence was 29.4% in the 15-19 years-group, decreasing progressively to 6.1% at 50-54 years of age and increasing to 12.2% in those aged over 65 years. HPV 16 was the genotype detected most frequently followed by HPV 31, HPV 18, HPV 56, and HPV 51. HPV 16 or 18 were present in 4% of women with normal cytology and both were detected contemporarily in only 14 women. Twenty-two percent of atypical squamous cells, 26% of low-grade and 56% of high-grade squamous intraepithelial lesions at cytology were positive for HPV 16 and/or 18. The prevalence of HPV infection in Italy is in agreement with that reported worldwide. HPV 16 was the prevalent genotype. The concomitant infection with HPV 16 and HPV 18 (vaccine targets) was found rarely. Apart from HPV 16 and 18, there was a substantial presence of HPV genotypes against which the vaccines available currently have shown cross-protection efficacy. The findings of this study may contribute to reliable predictions on the potential efficacy of an HPV vaccine in clinical practice.

Agarossi A ，Ferrazzi E ，Parazzini F ，Perno CF ，Ghisoni L ... -  《-》

Sexual habits and human papillomavirus infection among females in three New Independent States of the former Soviet Union.

Syrjänen S ，Shabalova I ，Petrovichev N ，Kozachenko V ，Zakharova T ，Pajanidi J ，Podistov J ，Chemeris G ，Sozaeva L ，Lipova E ，Tsidaeva I ，Ivanchenko O ，Pshepurko A ，Zakharenko S ，Nerovjna R ，Kljukina L ，Erokhina O ，Branovskaja M ，Nikitina M ，Grujnberga V ，Grujnberg A ，Juschenko A ，Johansson B ，Tosi P ，Cintorino M ，Santopietro R ，Syrjänen K ... -  《SEXUALLY TRANSMITTED DISEASES》

Prevalence and genotype distribution of multiple human papillomavirus infection in the uterine cervix: a 7.5-year longitudinal study in a routine cytology-based screening population in West Germany.

Kovács K ，Varnai AD ，Bollmann M ，Bankfalvi A ，Szendy M ，Speich N ，Schmitt C ，Pajor L ，Bollmann R ... -  《-》

[Detection and typing of human papilloma virus by polymerase chain reaction and hybridization assay in cervical samples with cytological abnormalities].

Ergünay K ，Misirlioğlu M ，Firat P ，Tuncer ZS ，Tuncer S ，Yildiz I ，Ustaçelebi S ... -  《MIKROBIYOLOJI BULTENI》