Quantification of trandolapril and its metabolite trandolaprilat in human plasma by liquid chromatography/tandem mass spectrometry using solid-phase extraction.

A sensitive high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (MS/MS) method was developed and validated for the simultaneous quantification of trandolapril and its metabolite trandolaprilat in human plasma using ramipril as an internal standard. Following solid-phase extraction, the analytes were separated using an isocratic mobile phase on a reversed-phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M-H]- ions, m/z 429/168 for trandolapril, m/z 401/168 for trandolaprilat and m/z 415/166 for the internal standard. The method exhibited a linear dynamic range of 20-10,000 pg/mL for both trandolapril and trandolaprilat in human plasma. The lower limit of quantification was 20 pg/mL for both trandolapril and its metabolite. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.0 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies.

Nirogi RV ，Kandikere VN ，Shrivastava W ，Mudigonda K ... -  《RAPID COMMUNICATIONS IN MASS SPECTROMETRY》

Quantification of tizanidine in human plasma by liquid chromatography coupled to tandem mass spectrometry.

Nirogi RV ，Kandikere VN ，Shukla M ，Mudigonda K ，Maurya S ... -  《RAPID COMMUNICATIONS IN MASS SPECTROMETRY》

Quantification of granisetron in human plasma by liquid chromatography coupled to electrospray tandem mass spectrometry.

A simple, sensitive and rapid high-performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the assay of granisetron in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reversed-phase C18 column and analyzed by MS in the multiple reaction monitoring mode using the respective [M+H]+ ions, m/z 313/138 for granisetron and m/z 409/228 for the IS. The assay exhibited a linear dynamic range of 0.1-20 ng/mL for granisetron in human plasma. The lower limit of quantification was 100 pg/mL with a relative standard deviation of less than 5%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.0 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies.

Nirogi RV ，Kandikere VN ，Shukla M ，Mudigonda K ，Maurya S ，Boosi R ... -  《BIOMEDICAL CHROMATOGRAPHY》

Quantification of pseudoephedrine in human plasma by LC-MS/MS using mosapride as internal standard.

Nirogi RV ，Kandikere VN ，Shukla M ，Mudigonda K ，Maurya S ，Komarneni P ... -  《BIOMEDICAL CHROMATOGRAPHY》

Liquid chromatography tandem mass spectrometry method for the quantification of clonidine with LLOQ of 10 pg/mL in human plasma.

Nirogi R ，Kandikere V ，Mudigonda K ，Komarneni P ... -  《BIOMEDICAL CHROMATOGRAPHY》