Quantitative analyses of indoloquinazoline alkaloids in Fructus Evodiae by high-performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry.

Fructus Evodiae (Wuzhuyu), the fruits of Evodia rutaecarpa and related varieties, is widely used in traditional Chinese medicine. The bioactive constituents include the indoloquinazoline alkaloids rutaecarpine, evodiamine and dehydroevodiamine. A new assay based on high-performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/UV/APCI-MS/MS) was developed for the measurement of the indoloquinazoline alkaloids in commercial Fructus Evodiae products. Initially, the MS/MS fragmentation pathways of indoloquinazoline alkaloids were investigated to identify fragment ions that might be useful for the sensitive and selective detection of trace indoloquinazoline alkaloids during LC/MS/MS. Then, quantitative MS analysis of five indoloquinazoline alkaloids in 12 commercial Fructus Evodiae products from different geographical sources was performed. Analyte recovery was in the range of 97.5-105.3% for all with relative standard deviations (RSDs) below 6%, the intra-assay and inter-assay RSDs were less than 7%, and good linear relationships were shown with correlation coefficients for the analytes exceeding 0.999. Therefore, this LC/MS/MS assay facilitated the rapid quantitative analysis of rutaecarpine, evodiamine, evodiamide, 14-formyldihydrorutaecarpine and dehydroevodiamine in 12 commercial Fructus Evodiae products with excellent recovery, repeatability, accuracy and sensitivity. This method is simple and specific and can be used for identification and quality control of this traditional Chinese remedy.

Zhou Y ，Li SH ，Jiang RW ，Cai M ，Liu X ，Ding LS ，Xu HX ，But PP ，Shaw PC ... -  《RAPID COMMUNICATIONS IN MASS SPECTROMETRY》

Gram-scale purification of dehydroevodiamine from Evodia rutaecarpa fruits, and a procedure for selective removal of quaternary indoloquinazoline alkaloids from Evodia extracts.

Dehydroevodiamine (DHE) is a major bioactive constituent in the traditional Chinese herbal drug Evodiae fructus (Wu zhu yu). The compound has been shown to possess pronounced cardiovascular and neuropharmacological activities in vitro and in vivo. For quality control purposes and follow-up studies assessing potential safety risks of DHE, we developed a simple and efficient two-step protocol for gram-scale purification of DHE. An alkaloidal fraction was obtained by cation-exchange solid phase extraction, and DHE and the minor alkaloid hortiamine were purified by isocratic preparative RP-HPLC. The DHE content in different commercial batches of Evodiae fructus, and in a series of commercially available Evodia-containing TCM products was assessed. A daily intake of up to mg amounts of DHE was calculated from recommended doses of these products. A method for the selective removal of quaternary indoloquinazoline alkaloids from Evodia extracts was developed.

Schramm A ，Hamburger M 《-》

Quality evaluation of Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography with photodiode-array detection.

Zhao Y ，Li Z ，Zhou X ，Cai Z ，Gong X ，Zhou C ... -  《JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS》

Simultaneous determination of six bioactive compounds in Evodiae Fructus by high-performance liquid chromatography with diode array detection.

Tang X ，Huang Z ，Chen Y ，Liu Y ，Liu Y ，Zhao J ，Yi J ... -  《-》

[Mass spectrometric analysis of quinazoline alkaloid and limonin from Fructus Evodiae].

Liao QF ，Xie ZY ，Zhang L ，Deng YT ，Li C ，Wei CF ，Yao Y ... -  《-》