Remodeling of extracellular matrix at ovulation of the bovine ovarian follicle.

Using immunohistochemistry and RNA analyses we examined the fate of components of a newly identified matrix that develops between granulosa cells (focimatrix, abbreviated from focal intraepithelial matrix) and of the follicular basal lamina in ovulating bovine ovarian follicles. Pre- and postovulatory follicles were generated by treatment with estradiol (Day 1), progesterone (Days 1-10), and prostaglandin analogue (Day 9) with either no further treatment (Group 1, n = 6) and or with 25 mg porcine LH (Day 11, Group 2, n = 8 or Day 10, Group 3, n = 8) and ovariectomy on Day 12 (12-14 hr post LH in Group 2, 38-40.5 hr in Group 3). In the time frame examined no loss of follicular basal lamina laminin chains beta2 and gamma1 or nidogen 1 was observed. In the follicular basal lamina collagen type IV alpha1 and perlecan were present prior to ovulation; after ovulation collagen type IV alpha1 was discontinuously distributed and perlecan was absent. Versican in the theca interna adjacent to the follicular basal lamina in preovulatory follicles was not observed post ovulation, however, the granulosa cells then showed strong cytoplasmic staining for versican. Expression of versican isoforms V0, V1, and V3 was detected at all stages. Focimatrix was observed in preovulatory follicles. It contained collagen type IV alpha1, laminins beta2 and gamma1, nidogen 1 and perlecan and underwent changes in composition similar to that of the follicular basal lamina. In conclusion focimatrix and the follicular basal lamina are degraded at ovulation. Individual components are lost at different times.

Irving-Rodgers HF ，Catanzariti KD ，Aspden WJ ，D'Occhio MJ ，Rodgers RJ ... -  《MOLECULAR REPRODUCTION AND DEVELOPMENT》

A novel basal lamina matrix of the stratified epithelium of the ovarian follicle.

Irving-Rodgers HF ，Harland ML ，Rodgers RJ 《MATRIX BIOLOGY》

Extracellular matrix of the developing ovarian follicle.

Irving-Rodgers HF ，Rodgers RJ 《-》

Basement membrane composition in the early mouse embryo day 7.

Gersdorff N ，Müller M ，Otto S ，Poschadel R ，Hübner S ，Miosge N ... -  《DEVELOPMENTAL DYNAMICS》

Studies of granulosa cell maturation in dominant and subordinate bovine follicles: novel extracellular matrix focimatrix is co-ordinately regulated with cholesterol side-chain cleavage CYP11A1.

Irving-Rodgers HF ，Harland ML ，Sullivan TR ，Rodgers RJ ... -  《-》