Characterization of TGF-beta-regulated interleukin-8 expression in human prostate cancer cells.

Interleukin (IL)-8 and transforming growth factor (TGF)-beta1 are overexpressed in advanced prostate cancer. The purpose of this study was to investigate TGF-beta1-regulated IL-8 expression in prostate cancer cells. TGF-beta receptor expression was evaluated by real-time reverse-transcription PCR (RT-PCR) and Western blotting. TGF-beta1-regulated IL-8 expression was determined by real-time RT-PCR, enzyme-linked immunoabsorbance assay (ELISA), nuclear run-on, and IL-8 promoter reporter assay. PC-3MM2 cells expressed type I and type II TGF-beta receptors (TbetaRI and TbetaRII). LNCaP cells expressed significantly lower level of TbetaRII. Constitutive expression of IL-8 was detected in PC-3MM2 cells and LNCaP cells engineered with TbetaRII (LNCaP-TbetaRII). TGF-beta1 stimulated IL-8 expression in dose- and time-dependent manners, which was blocked by cycloheximide (CHX) and actinomycin D (ActD). The nuclear run-on and IL-8 luciferase reporter assays show that TGF-beta1 activated IL-8 gene transcription. TGF-beta1 signaling regulates IL-8 expression in prostate cancer cells and may contribute to the overexpression of IL-8 in human prostate cancer.

Lu S ，Dong Z 《PROSTATE》

Blockade of transforming growth factor-beta signaling suppresses progression of androgen-independent human prostate cancer in nude mice.

Zhang F ，Lee J ，Lu S ，Pettaway CA ，Dong Z ... -  《CLINICAL CANCER RESEARCH》

Regulation of TGF-beta1 gene transcription in human prostate cancer cells by nitric oxide.

Wang D ，Lu S ，Dong Z 《PROSTATE》

Restoration of transforming growth factor beta signaling pathway in human prostate cancer cells suppresses tumorigenicity via induction of caspase-1-mediated apoptosis.

Guo Y ，Kyprianou N 《CANCER RESEARCH》

The conventional transforming growth factor-beta (TGF-beta) receptor type I is not required for TGF-beta 1 signaling in a human prostate cancer cell line, LNCaP.

Kim IY ，Zelner DJ ，Lee C 《EXPERIMENTAL CELL RESEARCH》