Antibacterial effects of human group IIA and group XIIA phospholipase A2 against Helicobacter pylori in vitro.

Group IIA phospholipase A2 (PLA2-IIA) is an enzyme which has important roles in inflammation and infection. Recently, a novel human secretory PLA2 called group XIIA PLA2 (PLA2-XIIA) has been identified. Both PLA2-IIA and PLA2-XIIA are bactericidal against Gram-positive bacteria like many other secretory PLA2s. However, PLA2-XIIA is the only known PLA2 displaying significant bactericidal activity against the Gram-negative bacterium Escherichia coli. We examined the antibacterial properties of recombinant human PLA2-IIA and PLA2-XIIA against Helicobacter pylori, a Gram-negative bacterium, in vitro. PLA2-IIA was not bactericidal against H. pylori, whereas PLA2-XIIA effectively killed H. pylori at a concentration of 50 microg/ml but was not bactericidal at concentrations of 0.5 microg/ml and 5 microg/ml.

Huhtinen HT ，Grönroos JO ，Grönroos JM ，Uksila J ，Gelb MH ，Nevalainen TJ ，Laine VJ ... -  《APMIS》

Mobilization of potent plasma bactericidal activity during systemic bacterial challenge. Role of group IIA phospholipase A2.

Weinrauch Y ，Abad C ，Liang NS ，Lowry SF ，Weiss J ... -  《JOURNAL OF CLINICAL INVESTIGATION》

Bactericidal group IIA phospholipase A2 in serum of patients with bacterial infections.

Grönroos JO ，Laine VJ ，Nevalainen TJ 《JOURNAL OF INFECTIOUS DISEASES》

Bactericidal properties of group IIA and group V phospholipases A2.

:Group V phospholipase A(2) (PLA(2)) is a recently characterized 14-kDa secretory PLA(2) of mammalian heart and macrophage-derived cells. Group IIA PLA(2), which is structurally close to group V PLA(2), has been shown to kill Gram-positive bacteria in vitro and to prevent symptoms of Gram-positive infection in vivo. We studied the antibacterial properties of fully active recombinant rat group IIA and V PLA(2)s. Both group IIA and V PLA(2)s were highly bactericidal against Gram-positive bacteria, including methicillin-resistant staphylococci and vancomycin-resistant enterococci. Only high concentrations of group IIA PLA(2) showed some bactericidal effect against the Gram-negative bacterium Escherichia coli. Our results confirm that group IIA PLA(2) is a potent antibacterial enzyme against Gram-positive bacteria. Moreover, we show here that group V PLA(2) is a novel antibacterial mammalian protein, but is less potent than group IIA PLA(2). Both enzymes may be considered as future therapeutic agents against bacterial infections.

Grönroos JO ，Laine VJ ，Janssen MJ ，Egmond MR ，Nevalainen TJ ... -  《JOURNAL OF IMMUNOLOGY》

Cell-wall determinants of the bactericidal action of group IIA phospholipase A2 against Gram-positive bacteria.

Foreman-Wykert AK ，Weinrauch Y ，Elsbach P ，Weiss J ... -  《JOURNAL OF CLINICAL INVESTIGATION》