Strategies to develop chimerism in vascularized skin allografts across MHC barrier.

In this study, we investigated the effects of 7-day-protocols of alphabeta-T-cell receptor monoclonal antibody (alphabeta-TCRmAb), cyclosporine A (CsA), and tacrolimus (FK-506) immunosuppressive monotherapies, and their combinations on the survival of vascularized skin allografts (VSA). Forty-two transplantations of VSA across a strong MHC barrier were performed between ACI (RT1a) donors and Lewis (RT1(l)) recipients in seven groups. Isograft and allograft rejection controls received no treatment. Treatment groups received a 7-day protocol of alphabeta-TCRmAb, CsA, or FK-506 monotherapy, or a combination of alphabeta-TCRmAb/CsA and alphabeta-TCRmAb/FK-506. VSA transplants were evaluated on a daily basis. Donor-specific chimerism was determined by flow cytometry (FC). The combined protocols of alphabeta-TCRmAb/FK-506 and alphabeta-TCRmAb/CsA significantly prolonged VSA survivals compared to monotherapy groups ( P < 0.005). FC analysis revealed 15.82% of donor-specific chimerism on day 7 under the alphabeta-TCRmAb/CsA protocol and a gradual chimerism decline on day 63 posttransplant. The significant extension of VSA survival achieved under 7-day protocols of combined therapies was directly associated with the presence of donor-specific chimerism.

Demir Y ，Ozmen S ，Klimczak A ，Mukherjee AL ，Siemionow MZ ... -  《MICROSURGERY》

Donor-specific tolerance in fully major histocompatibility major histocompatibility complex-mismatched limb allograft transplants under an anti-alphabeta T-cell receptor monoclonal antibody and cyclosporine A protocol.

Siemionow MZ ，Izycki DM ，Zielinski M 《TRANSPLANTATION》

Applications of bilateral vascularized femoral bone marrow transplantation for chimerism induction across the major histocompatibility (MHC) barrier: part II.

Klimczak A ，Agaoglu G ，Carnevale KA ，Siemionow M ... -  《ANNALS OF PLASTIC SURGERY》

Trafficking of donor-derived bone marrow correlates with chimerism and extension of composite allograft survival across MHC barrier.

Ozmen S ，Ulusal BG ，Ulusal AE ，Izycki D ，Yoder B ，Siemionow M ... -  《TRANSPLANTATION PROCEEDINGS》

Role of thymus in operational tolerance induction in limb allograft transplant model.

Siemionow M ，Izycki D ，Ozer K ，Ozmen S ，Klimczak A ... -  《TRANSPLANTATION》