Evaluation of the BD Phoenix Automated Microbiology System for identification and antimicrobial susceptibility testing of common clinical isolates.

To evaluate the accuracy and reliability of the BD Phoenix Automated Microbiology System for identification (ID) and antimicrobial susceptibility testing (AST) of Gram-positive and Gram-negative isolates. The ID of 291 Gram-negative and 158 Gram-positive clinical isolates were evaluated by the system. The AST of 252 Gram-negative and 151 Gram-positive isolates with correct ID were then evaluated. The results were compared with those of the API ID system and the microbroth dilution method. All discrepant results were repeated for verification. Over 90% Gram-negative and Gram-positive isolates were correctly identified to species or genus level by the Phoenix system. The minimum inhibitory concentration agreement rates between the Phoenix and the microbroth dilution methods within +/-1 dilution ranged from 87.3 to 97.6% for Gram-negative isolates, and from 50 to 100% for Gram-positive isolates. For Gram-positive isolates, vancomycin had significant low minimum inhibitory concentration agreement rates. Overall, the results indicate that the Phoenix system is a reliable system. It could provide accurate ID and AST results for routine clinical laboratories.

Liu ZK ，Ling TK ，Cheng AF 《MEDICAL PRINCIPLES AND PRACTICE》

Evaluation of the Phoenix 100 ID/AST system and NID panel for identification of Enterobacteriaceae, Vibrionaceae, and commonly isolated nonenteric gram-negative bacilli.

O'Hara CM 《-》

Two-center collaborative evaluation of performance of the BD phoenix automated microbiology system for identification and antimicrobial susceptibility testing of gram-negative bacteria.

Menozzi MG ，Eigner U ，Covan S ，Rossi S ，Somenzi P ，Dettori G ，Chezzi C ，Fahr AM ... -  《-》

Direct inoculation method using BacT/ALERT 3D and BD Phoenix System allows rapid and accurate identification and susceptibility testing for both Gram-positive cocci and Gram-negative rods in aerobic blood cultures.

This study describes a direct inoculation method using the automated BacT/ALERT 3D and the BD Phoenix System in combination for identification and susceptibility testing of isolates from positive blood cultures. Organism identification and susceptibility results were compared with the conventional method for 211 positive aerobic blood cultures. Of 110 Gram-positive cocci (GPCs), 98 (89.1%) isolates were correctly identified to the species level. Of 101 Gram-negative rods (GNRs), 98 (97.0%) isolates were correctly identified to the species level. The overall categorical agreement in antimicrobial susceptibility testing among the 110 GPCs was 92.7%, with 0.04% very major and 0.7% major error rates. The overall categorical agreement among 78 isolates of enterobacteria and 23 isolates of nonfermenters in GNRs was 99.5% and 91.1%, respectively, with no major errors identified. We conclude that, compared with previously reported direct inoculation methods, our method is superior in identification and susceptibility testing of GPCs.

Yonetani S ，Okazaki M ，Araki K ，Makino H ，Fukugawa Y ，Okuyama T ，Ohnishi H ，Watanabe T ... -  《-》

Evaluation of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of Enterobacteriaceae.

Carroll KC ，Glanz BD ，Borek AP ，Burger C ，Bhally HS ，Henciak S ，Flayhart D ... -  《-》