Identification of a sperm protein 17 CTL epitope restricted by HLA-A1.

Sperm protein 17 (Sp17) is a novel cancer-testis antigen. We previously reported the successful generation of Sp17-specific HLA-A1-restricted cytotoxic T-lymphocytes (CTLs) from the peripheral blood of a healthy donor using a recombinant Sp17 protein. These CTLs were able to kill not only target cells pulsed with the recombinant protein but also fresh Sp17+ tumor cells. In the present study, we have identified a nonapeptide sequence within the Sp17 protein that is predicted to have a high binding affinity for the HLA-A1 molecules. We generated the synthetic nonapeptide and successfully propagated a peptide-specific CTL line. We confirmed that peptide Sp17(103-111) (ILDSSEEDK) contains an Sp17 CTL epitope restricted by HLA-A1 and identified amino acid residues 104, 107, 109 and 110 as crucial for the CTL lysis. Our findings therefore provide the tool for the characterization of CD8 T-cell function in vivo and generation of epitope-specific treatment strategies.

Chiriva-Internati M ，Wang Z ，Pochopien S ，Salati E ，Lim SH ... -  《INTERNATIONAL JOURNAL OF CANCER》

Successful generation of sperm protein 17 (Sp17)-specific cytotoxic T lymphocytes from normal donors: implication for tumour-specific adoptive immunotherapy following allogeneic stem cell transplantation for Sp17-positive multiple myeloma.

Chiriva-Internati M ，Wang Z ，Salati E ，Wroblewski D ，Lim SH ... -  《SCANDINAVIAN JOURNAL OF IMMUNOLOGY》

Identification of new melanoma epitopes on melanosomal proteins recognized by tumor infiltrating T lymphocytes restricted by HLA-A1, -A2, and -A3 alleles.

Kawakami Y ，Robbins PF ，Wang X ，Tupesis JP ，Parkhurst MR ，Kang X ，Sakaguchi K ，Appella E ，Rosenberg SA ... -  《JOURNAL OF IMMUNOLOGY》

Sperm protein 17 (Sp17) in multiple myeloma: opportunity for myeloma-specific donor T cell infusion to enhance graft-versus-myeloma effect without increasing graft-versus-host disease risk.

Chiriva-Internati M ，Wang Z ，Xue Y ，Bumm K ，Hahn AB ，Lim SH ... -  《EUROPEAN JOURNAL OF IMMUNOLOGY》

Human melanoma patients recognize an HLA-A1-restricted CTL epitope from tyrosinase containing two cysteine residues: implications for tumor vaccine development.

Kittlesen DJ ，Thompson LW ，Gulden PH ，Skipper JC ，Colella TA ，Shabanowitz J ，Hunt DF ，Engelhard VH ，Slingluff CL Jr ... -  《JOURNAL OF IMMUNOLOGY》