Consistent immunohistochemical detection of intracellular beta-amyloid42 in pyramidal neurons of Alzheimer's disease entorhinal cortex.

We compared the effects of three pretreatment immunohistochemical techniques (no pretreatment, pepsin predigestion and heat pretreatment (HEAT)) for detecting intracellular beta-amyloid42 (Abeta42) in pyramidal neurons of formalin-fixed Alzheimer's disease (AD) cortices (n = 25). Although all three protocols immunostained Abeta42 in amyloid plaques using four commercially-obtained Abeta42 specific antibodies, only the HEAT protocol consistently detected prominent intracellular Abeta42 in pyramidal neurons. This suggests that the Abeta42 present in amyloid plaques may be structurally distinct from that located within the neurons perhaps due to differential binding proteins coupling or a consequence of formalin fixation. Detection of an abundant intracellular Abeta42 in neurons may provide alternate explanations for the origin of dense-core amyloid plaques in AD cortices other than the conventional chronic extracellular Abeta42 deposition hypothesis.

D'Andrea MR ，Nagele RG ，Wang HY ，Lee DH ... -  《NEUROSCIENCE LETTERS》

Lipofuscin and Abeta42 exhibit distinct distribution patterns in normal and Alzheimer's disease brains.

D'Andrea MR ，Nagele RG ，Gumula NA ，Reiser PA ，Polkovitch DA ，Hertzog BM ，Andrade-Gordon P ... -  《-》

Evidence that neurones accumulating amyloid can undergo lysis to form amyloid plaques in Alzheimer's disease.

D'Andrea MR ，Nagele RG ，Wang HY ，Peterson PA ，Lee DH ... -  《HISTOPATHOLOGY》

Targeting the alpha 7 nicotinic acetylcholine receptor to reduce amyloid accumulation in Alzheimer's disease pyramidal neurons.

D'Andrea MR ，Nagele RG 《CURRENT PHARMACEUTICAL DESIGN》

Intracellular accumulation of beta-amyloid(1-42) in neurons is facilitated by the alpha 7 nicotinic acetylcholine receptor in Alzheimer's disease.

Nagele RG ，D'Andrea MR ，Anderson WJ ，Wang HY ... -  《NEUROSCIENCE》