Effects of histone acetylation on transcriptional regulation of manganese superoxide dismutase gene.

To better understand the link between chromatin modification and manganese superoxide dismutase (Mn-SOD) gene expression, we have investigated the level of histone acetylation at Mn-SOD proximal promoter. TSA induced the expression of Mn-SOD mRNA and its transcriptional activity in C2C12 cells. Sp1 binding sites in the proximal promoter region of Mn-SOD were transcriptionally responsive to TSA by transfection studies. We have detected a localized acetylation of histones H3 and H4, in vivo occupation by Sp1, early growth responsive-1 (Egr-1), and histone deacetylase-1 (HDAC1) in the proximal promoter region of Mn-SOD gene using chromatin immunoprecipitation assays. Our findings indicate that Mn-SOD gene expression is repressed by Sp1-HDAC1 complex. This repression is released by a localized histone acetylation and at least in parts a displacement by Egr-1 in response to TSA.

Maehara K ，Uekawa N ，Isobe K 《BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS》

Sp1 and Sp3 transcription factors mediate trichostatin A-induced and basal expression of extracellular superoxide dismutase.

Zelko IN ，Folz RJ 《FREE RADICAL BIOLOGY AND MEDICINE》

The zinc finger repressor, ZBP-89, recruits histone deacetylase 1 to repress vimentin gene expression.

Wu Y ，Zhang X ，Salmon M ，Zehner ZE ... -  《GENES TO CELLS》

Coordinated changes in DNA methylation and histone modifications regulate silencing/derepression of luteinizing hormone receptor gene transcription.

Zhang Y ，Fatima N ，Dufau ML 《-》

Requirement of a specific Sp1 site for histone deacetylase-mediated repression of transforming growth factor beta Type II receptor expression in human pancreatic cancer cells.

Zhao S ，Venkatasubbarao K ，Li S ，Freeman JW ... -  《CANCER RESEARCH》