Sequence analysis of feline caliciviruses isolated from the oral cavity of clinically normal domestic cats (Felis catus) in Florida.

Four isolates (7.3 per cent) of feline calicivirus (FCV), from oropharyngeal swabs taken from 55 unvaccinated apparently healthy cats, were identified by electron microscopy and reverse transcription-polymerase chain reaction (RT-PCR). A 671-bp fragment, comprising part of region B, all of regions C, D, and E and part of region F of the FCV capsid protein gene, was amplified from each isolate by RT-PCR and cloned for sequence analysis. Amino acid sequence comparison of these regions revealed significant sequence divergence from the F9 vaccine strain within regions C, E, and F. The hypervariable region E of the four Florida isolates and the NADC isolate contained three fewer amino acids than the commonly used F9 vaccine strain. This work provides support to the idea that currently circulating FCV strains may differ substantially from presently used vaccine strains.

Weeks ML ，Gallagher A ，Romero CH 《RESEARCH IN VETERINARY SCIENCE》

Properties of a calicivirus isolated from cats dying in an agitated state.

Sato Y ，Ohe K ，Fukuyama M ，Furuhata K ，Kishikawa S ，Sakai S ，Kiuchi A ，Hara M ，Watanabe T ，Ishikawa Y ，Taneno A ... -  《VETERINARY RECORD》

Genetic and phylogenetic analyses of capsid protein gene in feline calicivirus isolates from Rio Grande do Sul in southern Brazil.

Feline calicivirus (FCV) is an important pathogen that affects domestic cats, inducing acute oral and upper respiratory tract clinical signs. The aim of this study was to analyze the variability of the capsid protein in different FCV isolates from southern Brazil. The sequencing analyses of thirteen Brazilian FCV samples, phylogenetic analyses and assessments of ten previously published sequences were conducted by examining the open reading frame 2 (ORF2, regions B-F). Comparisons of the predicted amino acid sequences of the ORF2 in Brazilian FCV isolates with those of the FCV-F9 strain indicated that the main differences are located within the regions C and hypervariable E (HVR_E). Epitopes that were mapped to the regions D, 5'HVR_E and conserved E also presented with some variability when compared to the strain F9. This is the first study describing sequence analyses and the phylogenetic relationships among FCV isolates from Brazil. The results presented here may expand upon current knowledge regarding aspects of FCV biology, epidemiology and genetic diversity and provide insights into improving the efficacies of current FCV vaccines.

Henzel A ，Sá e Silva M ，Luo S ，Lovato LT ，Weiblen R ... -  《-》

Detection and strain differentiation of feline calicivirus in conjunctival swabs by RT-PCR of the hypervariable region of the capsid protein gene.

Sykes JE ，Studdert VP ，Browning GF 《ARCHIVES OF VIROLOGY》

Real-time reverse transcription polymerase chain reaction assay to detect a broad range of feline calicivirus isolates.

Wilhelm S ，Truyen U 《JOURNAL OF VIROLOGICAL METHODS》