Cell cycle regulation via p53 phosphorylation by a 5'-AMP activated protein kinase activator, 5-aminoimidazole- 4-carboxamide-1-beta-D-ribofuranoside, in a human hepatocellular carcinoma cell line.

5-Aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) is an activator of AMP activated protein kinase (AMPK) and a regulator of de novo purine synthesis. There are several earlier reports indicating that AICAR treatment suppresses cell growth via regulation of AMPK or de novo purine synthesis. We found cell growth to be suppressed by AICAR treatment in HepG2 because of p53 accumulation, which was associated with p53-Ser15 phosphorylation. Moreover, a motif very similar to the consensus motif of AMPK phosphorylation was found around p53-Ser15, and Ser15 phosphorylation was detected in AICAR treated HepG2 as was in vitro phosphorylation by AMPK. Our results suggest that AICAR may regulate cell growth via p53 phosphorylation, and also indicate the possibility of p53 phosphorylation.

Imamura K ，Ogura T ，Kishimoto A ，Kaminishi M ，Esumi H ... -  《BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS》

5-Aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside stimulates tyrosine hydroxylase activity and catecholamine secretion by activation of AMP-activated protein kinase in PC12 cells.

Fukuda T ，Ishii K ，Nanmoku T ，Isobe K ，Kawakami Y ，Takekoshi K ... -  《JOURNAL OF NEUROENDOCRINOLOGY》

Overexpression of CYP2E1 induces HepG2 cells death by the AMP kinase activator 5'-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR).

Zhuge J 《-》

Activation of adenosine monophosphate activated protein kinase inhibits growth of multiple myeloma cells.

Baumann P ，Mandl-Weber S ，Emmerich B ，Straka C ，Schmidmaier R ... -  《EXPERIMENTAL CELL RESEARCH》

AMP-activated protein kinase activators can inhibit the growth of prostate cancer cells by multiple mechanisms.

Xiang X ，Saha AK ，Wen R ，Ruderman NB ，Luo Z ... -  《BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS》