Synthesis and protein distribution of the unspliced large tenascin-C isoform in oral squamous cell carcinoma.

The inclusion or omission of the alternatively spliced region in the tenascin-C (Tn-C) mRNA gives rise to the large (Tn-C(L)) or small (Tn-C(S)) variant, respectively. Tn-C(L) is thought to be a typical component of provisional extracellular matrices (ECMs) and is expressed during tumour stroma remodelling. Tn-C(L) synthesis has been studied using RNA/RNA in situ hybridization, and Tn-C(L) protein distribution, using immunohistochemistry (clone BC-2), in 18 oral squamous cell carcinomas (OSCCs) of different grades of malignancy. While the Tn-C(L) protein was demonstrated within the whole stromal compartment regardless of grade of malignancy, the majority of the Tn-C(L) mRNA signal-bearing cells were carcinoma cells. Only a few stromal myofibroblasts were able to synthesize Tn-C(L), as revealed by alpha-smooth muscle actin double staining. In well-differentiated carcinomas (G1), the Tn-C(L) synthesizing carcinoma cells were localized as a single positive cell layer in the tumour stroma interface, particularly in invasive areas. A higher grade of malignancy (G2/G3) is associated with a significantly increased number of Tn-C(L) synthesizing carcinoma cells randomly distributed within the invading tumour areas. Double-staining experiments (Tn-C(L) mRNA ISH/BC-2 immunohistochemistry) indicate that these cells are capable of organizing and depositing a three-dimensional Tn-C(L) matrix. Even though an instructive and/or inductive role of the carcinoma cells in tumour stroma formation cannot be excluded, these results demonstrate that carcinoma cells can directly produce the ECM components of tumour stroma.

Hindermann W ，Berndt A ，Borsi L ，Luo X ，Hyckel P ，Katenkamp D ，Kosmehl H ... -  《JOURNAL OF PATHOLOGY》

mRNA expression and protein distribution of the unspliced tenascin-C isoform in prostatic adenocarcinoma.

Katenkamp K ，Berndt A ，Hindermann W ，Wunderlich H ，Haas KM ，Borsi L ，Zardi L ，Kosmehl H ... -  《JOURNAL OF PATHOLOGY》

Distribution of laminin and fibronectin isoforms in oral mucosa and oral squamous cell carcinoma.

Kosmehl H ，Berndt A ，Strassburger S ，Borsi L ，Rousselle P ，Mandel U ，Hyckel P ，Zardi L ，Katenkamp D ... -  《-》

A quantitative co-localization analysis of large unspliced tenascin-C(L) and laminin-5/gamma2-chain in basement membranes of oral squamous cell carcinoma by confocal laser scanning microscopy.

Franz M ，Hansen T ，Borsi L ，Geier C ，Hyckel P ，Schleier P ，Richter P ，Altendorf-Hofmann A ，Kosmehl H ，Berndt A ... -  《JOURNAL OF ORAL PATHOLOGY & MEDICINE》

Tenascin-C expression and its prognostic significance in oral and pharyngeal squamous cell carcinoma.

Atula T ，Hedström J ，Finne P ，Leivo I ，Markkanen-Leppänen M ，Haglund C ... -  《ANTICANCER RESEARCH》