Chemotactic mediator requirements in lung injury following skin burns in rats.

:Partial-thickness skin burns have been shown to induce neutrophil-dependent microvascular injury both locally (skin) and systemically (lung). In the present study, interventional measures to block inflammatory chemoattractants were employed to define the pathophysiologic role of these mediators in the development of secondary lung injury following thermal injury of skin. Rats were treated with blocking antibodies to either C5a or to the alpha-chemokines, keratinocyte-derived cytokine (KC), or macrophage inflammatory protein-2 (MIP-2). To study the role of platelet activating factor, a receptor antagonist (PAF-Ra) was utilized. The development of lung vascular injury following thermal injury to skin was significantly attenuated by treatment with anti-C5a (84%), anti-KC (67%), and anti-MIP-2 (77%), but treatment with PAF-Ra had no protective effects. Protective interventions were paralleled by significant reductions in the tissue buildup of myeloperoxidase. When bronchoalveolar lavage fluids from thermally injured rats were evaluated, elevations in TNF;ZA and IL-1 were found and were determined to be C5a-dependent (but unaffected by treatment with PAF-Ra). These studies indicate that lung tissue injury after thermal skin burns is dependent on chemotactic mediators. The data also suggest that lung expression of TNFalpha and IL-1 after thermal injury of skin is C5a-dependent.

Piccolo MT ，Wang Y ，Sannomiya P ，Piccolo NS ，Piccolo MS ，Hugli TE ，Ward PA ，Till GO ... -  《EXPERIMENTAL AND MOLECULAR PATHOLOGY》

Requirement for C-X-C chemokines (macrophage inflammatory protein-2 and cytokine-induced neutrophil chemoattractant) in IgG immune complex-induced lung injury.

Shanley TP ，Schmal H ，Warner RL ，Schmid E ，Friedl HP ，Ward PA ... -  《JOURNAL OF IMMUNOLOGY》

In vitro and in vivo dependency of chemokine generation on C5a and TNF-alpha.

Czermak BJ ，Sarma V ，Bless NM ，Schmal H ，Friedl HP ，Ward PA ... -  《JOURNAL OF IMMUNOLOGY》

Role of chemotactic factors in neutrophil activation after thermal injury in rats.

Piccolo MT ，Wang Y ，Verbrugge S ，Warner RL ，Sannomiya P ，Piccolo NS ，Piccolo MS ，Hugli TE ，Ward PA ，Till GO ... -  《INFLAMMATION》

Divergent roles of murine neutrophil chemokines in hemorrhage induced priming for acute lung injury.

Lomas-Neira J ，Chung CS ，Grutkoski PS ，Dunican A ，Simms HH ，Cioffi WG ，Ayala A ... -  《CYTOKINE》