Protective effect of NK1.1(+) T cells as well as NK cells against intraperitoneal tumors in mice.

Peritoneal resident cells of mice normally contain small populations of NK cells and NK1.1(+) alphabetaT cells. These populations increased after either 3LL or EL4 tumor inoculations into the peritoneal cavity. In vivo depletion of NK cell alone by anti-asialo GM1 (ASGM1) Ab significantly decreased survival time of tumor-injected mice, while depletion of both NK cells and NK1.1(+) T cells by anti-NK 1.1 Ab greatly shortened mouse survival time. NK1. 1(+) T cells in peritoneal cavity consist of a larger proportion of double-negative T cells and smaller populations of CD4(+) T cells and Vbeta8(+) T cells compared with liver NK1.1(+) T cells and normally lack Vbeta2(+) T cells. Tumor inoculation induced rapid IL-12 and IFN-gamma mRNA in tumor-infiltrating mononuclear cells (TIM). Although anti-NK1 Ab pretreatment in vivo abrogated IFN-gamma mRNA expression and IFN-gamma production of TIM, NK cell depletion alone by anti-ASGM1 Ab pretreatment retained IFN-gamma mRNA expression and partly inhibited IFN-gamma production of TIM. Peritoneal NK cells as well as NK1.1(+) T cells but not NK1.1(-) T cells of 3LL cell- or EL4 cell-injected mice showed cytotoxicities against the same tumor cells. Further, either anti-IL-12 Ab or anti-IFN-gamma Ab ip injection significantly shortened EL4 cell-inoculated mouse survival time. Our findings suggest that peritoneal macrophages activated by tumors produce IL-12 which activates NK cells and NK1.1(+) T cells to produce IFN-gamma and both NK cells and NK1.1(+) T cells are important in suppressing the growth of the intraperitoneal tumors.

Kawamura T ，Seki S ，Takeda K ，Narita J ，Ebe Y ，Naito M ，Hiraide H ，Abo T ... -  《CELLULAR IMMUNOLOGY》

Activation of mouse liver natural killer cells and NK1.1(+) T cells by bacterial superantigen-primed Kupffer cells.

Dobashi H ，Seki S ，Habu Y ，Ohkawa T ，Takeshita S ，Hiraide H ，Sekine I ... -  《HEPATOLOGY》

Involvement of NK1+ T cells and their IFN-gamma production in the generalized Shwartzman reaction.

Ogasawara K ，Takeda K ，Hashimoto W ，Satoh M ，Okuyama R ，Yanai N ，Obinata M ，Kumagai K ，Takada H ，Hiraide H ，Seki S ... -  《JOURNAL OF IMMUNOLOGY》

Liver NK1.1+ CD4+ alpha beta T cells activated by IL-12 as a major effector in inhibition of experimental tumor metastasis.

Takeda K ，Seki S ，Ogasawara K ，Anzai R ，Hashimoto W ，Sugiura K ，Takahashi M ，Satoh M ，Kumagai K ... -  《JOURNAL OF IMMUNOLOGY》

Predominant appearance of NK1.1+ T cells producing IL-4 may be involved in the increased susceptibility of mice with the beige mutation during Salmonella infection.

Enomoto A ，Nishimura H ，Yoshikai Y 《JOURNAL OF IMMUNOLOGY》