自引率: 6.2%
被引量: 2487
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审稿周期: 5.17
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3 Biotech is a hybrid journal that publishes results of the latest research related to the study and application of biotechnology to Medicine and Biomedical Sciences, Agriculture and the Environment. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. <br /> <br />This journal is a suitable publication home for scientists and engineers in both academia and industry focusing on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment. <br /> <br />3 Biotech accepts original and review articles as well as short research reports, protocols and methods, notes to the editor, letters to the editor and book reviews for publication. Up to date, topical review articles will also be considered. All the manuscripts are peer-reviewed for scientific quality and acceptance.<br /><br />3 Biotech is indexed in Science Citation Index Expanded and range of Abstracting &Indexing services among which includes: Journal Citation Reports/Science Edition, Biological Abstracts, BIOSIS Previews and SCOPUS.
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Comparative miRNA signatures among Sahiwal and Frieswal cattle breeds during summer stress.
MicroRNAs (miRNAs) are known to take part in different biological mechanisms, including biotic as well as abiotic cellular stresses. The present investigation was aimed to identify comparative expression profile of differentially expressed miRNAs among Sahiwal () and Frieswal ( × ) cattle breeds during summer stress. Stress responses in animals were characterized by recording various physiological parameters, biochemical assays and expression profiling of heat shock protein 70 (Hsp70) during elevated environmental temperature. Ion Torrent-based deep sequencing as well as CLC-genomic analysis identified 322 and 420 annotated miRNAs among Sahiwal and Frieswal, respectively. A total 69 common miRNAs were identified to be differentially expressed during summer among the breeds. Out of the 69, a total 14 differentially expressed miRNAs viz. bta-mir 6536-2, bta-mir-2898, bta-mir-let-7b, bta-mir-425, bta-mir-2332, bta-mir-2478, bta-mir-150, bta-mir142, bta-mir-16a, bta-mir-2311, bta-mir-1839, bta-mir-1248-1, bta-mir-103-2 and bta-mir-181b were randomly selected for qRT-PCR-based validation. bta-mir-2898, bta-mir-6536-1, bta-mir-let-7b, bta-mir-2478, bta-mir-150, bta-mir-16a, bta-mir-2311, bta-mir-1032-b and bta-mir-181-b were significantly ( < 0.01) upregulated during summer among Frieswal in comparison to Sahiwal while, bta-mir 6536-2, bta-mir-2332, bta-mir142, bta-mir-1839 and bta-mir-1248-1 was significantly ( < 0.01) expressed at higher level in Sahiwal in contrast to Frieswal correlation coefficient analysis revealed that bta-mir(s)-150, 16a and 181b are negatively correlated ( < 0.05) with Hsp70 expression. Thus, this study identified that miRNA expression during summer stress can vary between the breeds which may reflect their differential post-transcriptional regulation. The online version contains supplementary material available at 10.1007/s13205-020-02608-4.
被引量：- 发表：1970
