Microbial Genomics
微生物基因组学
ISSN: 2057-5858
自引率: 暂无数据
发文量: 102
被引量: 1939
影响因子: 4.863
通过率: 暂无数据
出版周期: 月刊
审稿周期: 3
审稿费用: 0
版面费用: 1500
年文章数: 142
国人发稿量: 7

投稿须知/期刊简介:

Co-Editors-in-Chief: Professor Nicholas Thomson and Professor Stephen Bentley, Wellcome Trust Sanger Institute, UK Published by the Microbiology Society, Microbial Genomics is a Gold Open Access, peer-reviewed journal publishing cutting edge, pioneering research into genomic approaches to microbiology, fully supported by Open Data and innovative, collaborative services. We cover topics including, but not limited to: Microbial evolution (including viruses) Population genomics and phylogeography Outbreaks and epidemiological investigations Ecosystems, community and niche interactions Host pathogen, host commensal, host microbiota interactions The microbiome Responses to human interventions (e.g. clinical, agricultural) Metagenomic and whole transcriptome studies Functional genomics New models and computational methods Sequencing tools, algorithms and protocols We welcome articles showing novel insights, exciting new applications, or innovative approaches to analysis using genomic data, as well as articles developing our understanding of microbial genomics, from large, long-term studies on microbial evolution and epidemiology to studies that have immediate clinical or environmental relevance. We are particularly keen to encourage: Outbreak reports: genomic investigations of disease outbreaks that focus on the data and the process that led to the finding, as well as the finding itself, producing reproducible, robust research. Host-microbiota interactions, with a particular emphasis on studies where sequence data provides real biological insights into the role that microbial communities play in host health. Experimental evolution studies that focus on evolutionary responses to antimicrobial stresses, as well as those with longitudinal genomic data sets examining evolution in response to treatment and host-associated stresses. High-throughput imaging and metabolomics analysis of interactions and influences in both directions between microbe and host. Systems microbiology research using a hypothesis-driven, resource-aware approach combined with novel applications.   Microbial Genomics is indexed in Medline (PubMed), PubMed Central, Web of Science (Science Citation Index Expanded, Biological Abstracts and BIOSIS Previews), and Scopus, as well as on Google Scholar and ScienceOpen, ensuring maximum discoverability of your research.  

最新论文
  • Comparison of long-read sequencing technologies in the hybrid assembly of complex bacterial genomes.

    Illumina sequencing allows rapid, cheap and accurate whole genome bacterial analyses, but short reads (<300 bp) do not usually enable complete genome assembly. Long-read sequencing greatly assists with resolving complex bacterial genomes, particularly when combined with short-read Illumina data (hybrid assembly). However, it is not clear how different long-read sequencing methods affect hybrid assembly accuracy. Relative automation of the assembly process is also crucial to facilitating high-throughput complete bacterial genome reconstruction, avoiding multiple bespoke filtering and data manipulation steps. In this study, we compared hybrid assemblies for 20 bacterial isolates, including two reference strains, using Illumina sequencing and long reads from either Oxford Nanopore Technologies (ONT) or SMRT Pacific Biosciences (PacBio) sequencing platforms. We chose isolates from the family , as these frequently have highly plastic, repetitive genetic structures, and complete genome reconstruction for these species is relevant for a precise understanding of the epidemiology of antimicrobial resistance. We assembled genomes using the hybrid assembler Unicycler and compared different read processing strategies, as well as comparing to long-read-only assembly with Flye followed by short-read polishing with Pilon. Hybrid assembly with either PacBio or ONT reads facilitated high-quality genome reconstruction, and was superior to the long-read assembly and polishing approach evaluated with respect to accuracy and completeness. Combining ONT and Illumina reads fully resolved most genomes without additional manual steps, and at a lower consumables cost per isolate in our setting. Automated hybrid assembly is a powerful tool for complete and accurate bacterial genome assembly.

    被引量:120 发表:1970

  • Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches.

    This study aimed to assess the feasibility of using the Oxford Nanopore Technologies (ONT) MinION long-read sequencer in reconstructing fully closed plasmid sequences from eight isolates of six different species with plasmid populations of varying complexity. Species represented were , , , and , with plasmid populations ranging from 1-11 plasmids with sizes of 2-330 kb. Isolates were sequenced using Illumina (short-read) and ONT's MinION (long-read) platforms, and compared with fully resolved PacBio (long-read) sequence assemblies for the same isolates. We compared the performance of different assembly approaches including SPAdes, plasmidSPAdes, hybridSPAdes, Canu, Canu+Pilon (canuPilon) and npScarf in recovering the plasmid structures of these isolates by comparing with the gold-standard PacBio reference sequences. Overall, canuPilon provided consistently good quality assemblies both in terms of assembly statistics (N50, number of contigs) and assembly accuracy [presence of single nucleotide polymorphisms (SNPs)/indels with respect to the reference sequence]. For plasmid reconstruction, Canu recovered 70 % of the plasmids in complete contigs, and combining three assembly approaches (Canu or canuPilon, hybridSPAdes and plasmidSPAdes) resulted in a total 78 % recovery rate for all the plasmids. The analysis demonstrated the potential of using MinION sequencing technology to resolve important plasmid structures in species independent of and in conjunction with Illumina sequencing data. A consensus assembly derived from several assembly approaches could present significant benefit in accurately resolving the greatest number of plasmid structures.

    被引量:54 发表:1970

统计分析
是否有问题?您可以直接对期刊官方提问 提问

最近浏览

关于我们

zlive学术集成海量学术资源,融合人工智能、深度学习、大数据分析等技术,为科研工作者提供全面快捷的学术服务。在这里我们不忘初心,砥砺前行。

友情链接

联系我们

合作与服务

©2024 zlive学术声明使用前必读