自引率: 3%
被引量: 4666
通过率: 暂无数据
审稿周期: 暂无数据
版面费用: 暂无数据
国人发稿量: 10
投稿须知/期刊简介:
In January 2004, the journal Protein Engineering was relaunched as Protein Engineering, Design and Selection, or PEDS. PEDS publishes research papers and review articles relevant to the engineering, design and selection of proteins for use in biotechnology and therapy, and for understanding fundamental properties of activity, stability, folding, misfolding and disease. The journal has new editors, a new editorial structure, and a new reviewing system to make it a community journal run by protein scientists for their own discipline.
期刊描述简介:
A Community Based Journal run by Protein Scientists for Protein Scientists. Protein Engineering, Design and Selection (PEDS) publishes research papers and review articles relevant to the engineering, design and selection of proteins for use in biotechnology and therapy, and for understanding fundamental properties of activity, stability, folding, misfolding and disease. With reputable protein scientists as editors and board members, PEDS offers expert, high-quality peer review and a fast turnaround time.
-
Supercharged Phosphotriesterase for improved Paraoxon activity.
被引量：- 发表：2024
-
Engineered FHA domains can bind to a variety of Phosphothreonine-containing peptides.
被引量：- 发表：2024
-
An engineered NKp46 antibody for construction of multi-specific NK cell engagers.
Recent developments in cancer immunotherapy have highlighted the potential of harnessing natural killer (NK) cells in the treatment of neoplastic malignancies. Of these, bispecific antibodies, and NK cell engager (NKCE) protein therapeutics in particular, have been of interest. Here, we used phage display and yeast surface display to engineer RLN131, a unique cross-reactive antibody that binds to human, mouse, and cynomolgus NKp46, an activating receptor found on NK cells. RLN131 induced proliferation and activation of primary NK cells, and was used to create bispecific NKCE constructs of varying configurations and valency. All NKCEs were able to promote greater NK cell cytotoxicity against tumor cells than an unmodified anti-CD20 monoclonal antibody, and activity was observed irrespective of whether the constructs contained a functional Fc domain. Competition binding and fine epitope mapping studies were used to demonstrate that RLN131 binds to a conserved epitope on NKp46, underlying its species cross-reactivity.
被引量：- 发表：2024
-
Sequence-developability mapping of affibody and fibronectin paratopes via library-scale variant characterization.
Protein developability is requisite for use in therapeutic, diagnostic, or industrial applications. Many developability assays are low throughput, which limits their utility to the later stages of protein discovery and evolution. Recent approaches enable experimental or computational assessment of many more variants, yet the breadth of applicability across protein families and developability metrics is uncertain. Here, three library-scale assays-on-yeast protease, split green fluorescent protein (GFP), and non-specific binding-were evaluated for their ability to predict two key developability outcomes (thermal stability and recombinant expression) for the small protein scaffolds affibody and fibronectin. The assays' predictive capabilities were assessed via both linear correlation and machine learning models trained on the library-scale assay data. The on-yeast protease assay is highly predictive of thermal stability for both scaffolds, and the split-GFP assay is informative of affibody thermal stability and expression. The library-scale data was used to map sequence-developability landscapes for affibody and fibronectin binding paratopes, which guides future design of variants and libraries.
被引量：- 发表：2024
-
DexDesign: an OSPREY-based algorithm for designing de novo D-peptide inhibitors.
被引量：- 发表：2024
