自引率: 1.9%
被引量: 23124
通过率: 暂无数据
审稿周期: 暂无数据
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国人发稿量: 3
投稿须知/期刊简介:
Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by an editor who is an expert in the area covered, consists of invited articles. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.
期刊描述简介:
Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting. Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Some recent thematic issues covered these hot topics: • Chromatin Structure • Editing and investigating genomes with TALE and CRISPR • Tools and Methods for Cellular Localization and Measurements • Recent development in bioinformatics for utilizing omics data • Nucleic Acids Nanotechnology • Systems Biology with Omics Data • Autophagy • Spatial mapping of multi-modal data in neuroscience • Advancing the boundaries of molecular cellular pathology • NGS applications to translational research • Detection and Quantification of Proteins in Clinical Samples Using Mass Spectrometry
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HLA-DR4Pred2: An improved method for predicting HLA-DRB1*04:01 binders.
HLA-DRB1*04:01 is associated with numerous diseases, including sclerosis, arthritis, diabetes, and COVID-19, emphasizing the need to scan for binders in the antigens to develop immunotherapies and vaccines. Current prediction methods are often limited by their reliance on the small datasets. This study presents HLA-DR4Pred2, developed on a large dataset containing 12,676 binders and an equal number of non-binders. It's an improved version of HLA-DR4Pred, which was trained on a small dataset, containing 576 binders and an equal number of non-binders. All models were trained, optimized, and tested on 80 % of the data using five-fold cross-validation and evaluated on the remaining 20 %. A range of machine learning techniques was employed, achieving maximum AUROC of 0.90 and 0.87, using composition and binary profile features, respectively. The performance of the composition-based model increased to 0.93, when combined with BLAST search. Additionally, models developed on the realistic dataset containing 12,676 binders and 86,300 non-binders, achieved a maximum AUROC of 0.99. Our proposed method outperformed existing methods when we compared the performance of our best model to that of existing methods on the independent dataset. Finally, we developed a standalone tool and a webserver for HLADR4Pred2, enabling the prediction, design, and virtual scanning of HLA-DRB1*04:01 binding peptides, and we also released a Python package available on the Python Package Index (https://webs.iiitd.edu.in/raghava/hladr4pred2/; https://github.com/raghavagps/hladr4pred2; https://pypi.org/project/hladr4pred2/).
被引量:- 发表:1970
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Production, analysis, and safety assessment of a soil and plant-based natural material with microbiome- and immune-modulatory effects.
被引量:- 发表:1970
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AtML: An Arabidopsis thaliana root cell identity recognition tool for medicinal ingredient accumulation.
被引量:- 发表:1970
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A novel methodology for mapping interstitial fluid dynamics in murine brain tumors using DCE-MRI.
被引量:- 发表:1970
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Digital Intervention for behaviouR changE and Chronic disease prevenTION (DIRECTION): Study protocol for a randomized controlled trial of a web-based platform integrating nutrition, physical activity, and mindfulness for individuals with obesity.
被引量:- 发表:1970