自引率: 4.4%
被引量: 15595
通过率: 暂无数据
审稿周期: 2.65
版面费用: 暂无数据
国人发稿量: 93
投稿须知/期刊简介:
The Journal provides a medium for the rapid publication of both full-length articles and short communications on all aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome. The following is an outline of the areas covered in the Journal:Nucleic Acids/Molecular Biology: Novel contributions in the general area of Nucleic Acids/Molecular Biology will be considered. This includes studies for the physical and functional characterization of genomes, studies on the expression of genomic information in cellular and cell-free systems, the development and application of technologies for the detection of single molecules and molecular interactions (molecular recognition), the development and application of strategies towards the identification of biotechnologically interesting new compounds via chemical synthesis (combinatorial strategies in particular), molecular design and evolution, as well as molecular bioinformatics. The development of automated systems for the above mentioned fields may be of particular interest.Physiology/Biochemistry: This section covers biochemical and physiological studies of metabolism and enzymes as relevant to the product formation including intermediary metabolism of micro-organisms, tissue culture cells and cell-free systems; bioregulatory investigations at the molecular level including transciption/translation control and growth/product-synthesis relationships; design and engineering of products by molecular strategies with emphasis on protein/enzyme engineering and modification; quality improvement of non-protein products; engineering of cellular modification and transport systems such as post-translational protein modifications as well as protein and metabolite secretion; novel (molecular) strategies of screening for new or modified products (e.g. pharmaceuticals, bioactive compounds, enzymes) including application with product development based on enforced evolution and combinatorial strategies.Biochemical Engineering/Bioprocess Engineering: This section includes studies on transport phenomena, reaction kinetics, design of reactors downstream operations and software applications as well as research on cellular biology and physiology in biochemical processes employing enzymes, microorganisms, mammalian cells, plant cells and tissue. Of special interest is the rational manipulation of reactions through metabolic engineering techniques, the design of specific biocatalysts,
期刊描述简介:
The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome. The following areas are covered in the Journal: * Nucleic Acids/Molecular Biology * Physiology/Biochemistry * Biochemical Engineering/Bioprocess Engineering * Industrial Processes/New Products * Medical Biotechnology * Agro- and Food Biotechnology * Genomics and Bioinformatics
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Promotion of polyhydroxyalkanoates-producing granular sludge formation by lactic acid using anaerobic dynamic feeding process.
被引量：- 发表：1970
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Electrochemical biosensing of Acinetobacter baumannii gene using chitosan-gold composite modified electrode.
被引量：- 发表：1970
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Aminated lignin improved enzymatic hydrolysis of cellulosic substrate treated by p-toluenesulfonic acid.
Lignin can affect the enzymatic hydrolysis efficiency of lignocellulose. In this study, the lignin isolated from sugarcane bagasse (SCB) pretreated with p-toluenesulfonic acid (PL) was firstly aminated, and then the effects of PL and aminated PL (APL) on the bagasse enzymatic hydrolysis efficiency (EHE) were investigated. The results showed that the addition of PL and APL promoted the EHE, and EHE with APL (73.82 %) was higher than PL (51.39 %). To explore the reason, the data were further analyzed including cellulase adsorption capacity, enzyme activity, cellulase-lignin interaction, and molecular docking. It was found that APL adsorbed more cellulase (27.83 mg protein/g lignin) than PL (4.96 mg protein/g lignin), resulting from the greater interaction force and lower binding free energy between APL and cellulase. The addition of APL more remarkably enhanced the cellobiohydrolase and endoglucanase activities than PL due to more effectively inducing cellulase conformation optimization.
被引量：- 发表：1970
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Metabolic engineering of Escherichia coli for seleno-methylselenocysteine production.
Selenium (Se) is an essential trace element for life. Seleno-methylselenocysteine (SeMCys) can serve as a Se supplement with anticarcinogenic activity and can improve cognitive deficits. We engineered Escherichia coli for microbial production of SeMCys. The genes involved in the synthesis of SeMCys were divided into three modules-the selenocysteine (SeCys) synthesis, methyl donor synthesis and SMT modules-and expressed in plasmids with different copy numbers. The higher copy number of the SeCys synthesis module facilitated SeMCys production. The major routes for SeCys degradation were then modified. Deletion of the cysteine desulfurase gene csdA or sufS improved SeMCys production the most, and the strain that knocked out both genes doubled SeMCys production. The addition of serine in the mid-logarithmic growth phase significantly improved SeMCys synthesis. When the serine synthetic pathway was enhanced, SeMCys production increased by 12.5 %. Fed-batch culture for sodium selenite supplementation in the early stationary phase improved SeMCys production to 3.715 mg/L. This is the first report of the metabolic engineering of E. coli for the production of SeMCys and provide information on Se metabolism.
被引量：- 发表：1970
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Designing tailor-made steric matters to improve the immobilized ficin specificity for small versus large proteins.
The development of strategies that can permit to adjust the size specificity of immobilized proteases by the generation of steric hindrances may enlarge its applicability. Using as a model ficin immobilized on glyoxyl agarose, two strategies were assayed to generate tailor made steric hindrances. First, ficin has been coimmobilized on supports coated with large proteins (hemoglobin or bovine serum albumin (BSA)). While coimmobilization of ficin with BSA presented no effect on the activity versus any of the assayed substrates, coimmobilization with hemoglobin permitted to improve the immobilized ficin specificity for casein versus hemoglobin, but still significant activity versus hemoglobin remained. Second, aldehyde-dextran has been employed to modify the immobilized ficin, trying to generate steric hindrances to avoid the entry of large proteins (hemoglobin) while enabling the entry of small ones (casein). This also increased the size specificity of ficin, but still did not suppress the activity versus hemoglobin. The combination of both strategies and the use of 37ºC during the proteolysis enabled to almost fully nullify the hydrolytic activity versus hemoglobin while preserving a high percentage of the activity versus casein. The modifications improved enzyme stability and the biocatalyst could be reused for 5 cycles without alteration of its properties.
被引量：- 发表：1970
