自引率: 10.6%
被引量: 120695
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审稿周期: 4.6
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国人发稿量: 422
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Water Research publishes refereed, original research papers on all aspects of the science and technology of water quality and its management worldwide. A broad outline of the journal's scope includes: Treatment processes for water and wastewaters, municipal, agricultural and industrial, including residuals management. Water quality standards and the analysis, monitoring and assessment of water quality by chemical, physical and biological methods. Studies on inland, tidal or coastal waters, including surface and ground waters, and point and non-point sources of pollution. The limnology of lakes, impoundments and rivers. Solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions. Environmental restoration, including soil and groundwater remediation. Analysis of the interfaces between sediments and water, and water/atmosphere interactions. The application of mathematical and modelling techniques. Public health and risk assessment. Education and training.
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Use of a Salmonella typhimurium hilA fusion strain to assess effects of environmental fresh water sources on virulence gene expression.
Many fruits and vegetables are irrigated with water from rivers, lakes and even wastewater systems. Irrigation may be a route for the introduction of Salmonella. Our objectives in this study were to determine survivability and virulence expression in a strain of Salmonella typhimurium when exposed to environmental water sources. Virulence expression was measured using a beta-galactosidase assay on a hilA:lacZY fusion strain of S. typhimurium. Water samples for environmental impact studies were taken from a local pond and specific sites along the Rio Grande River, which serves as a source of irrigation water in southern Texas. There was a significant difference (p<0.05) of virulence expression among the water sites. Certain regions along the Rio Grande River yielded greater amounts of beta-galactosidase activity than others. All sites yielded at least a two-fold greater virulence response than S. typhimurium grown in brain heart infusion. Salmonella survivors were enumerated as colony forming units (CFU)/ml as plated on a selective medium for the duration of 1 week and beta-galactosidase assays were performed to determine a possible correlation between culturable cells and virulence gene expression. Bacterial cells remained viable but decreased after 7 days incubation. In conclusion, water sampled at specific locations and at different times water samples exhibited differences in virulence expression in S. typhimurium.
被引量：3 发表：2003
